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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/10/2023 |
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Data da última atualização: |
20/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. |
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Afiliação: |
CLEUDISON GABRIEL NASCIMENTO DA SILVA, UFRRJ; EDEVALDO DE CASTRO MONTEIRO, UFRRJ; PRISCILA PEREIRA DINIZ, UFRRJ; LEONARDO ARAUJO TERRA, UFRRJ; STEFAN SCHWAB, CNPAB; VERONICA MASSENA REIS, CNPAB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; SEGUNDO SACRAMENTO U CABALLERO, CNPAB. |
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Título: |
Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology,, Published: 16 October 2023 |
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ISSN: |
1678-4405 |
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Idioma: |
Inglês |
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Conteúdo: |
Endophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8 T ), Gluconacetobacter diazotrophicus (Pal5 T ), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specific primers for the real-time PCR (qPCR) assay. Primer specificity was confirmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the five target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specified in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplified target DNA fragments from each of the bacterial species, enabling their differentiation at the species level. The total bacterial population present in the sugarcane quantified using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes influence the endophytic population and the bacterial number decreases with plant age MenosEndophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8 T ), Gluconacetobacter diazotrophicus (Pal5 T ), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specific primers for the real-time PCR (qPCR) assay. Primer specificity was confirmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the five target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specified in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplified target DNA fragments from each of the bacterial species, enabling their differentiation at the species level. The total bacterial population present in the sugarcane quantified using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes... Mostrar Tudo |
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Palavras-Chave: |
Diazotrophic bacteria; DPGPB quantification; Primers; Real Time PCR. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02378naa a2200265 a 4500
001 2157398
005 2023-10-20
008 2023 bl uuuu u00u1 u #d
022 $a1678-4405
100 1 $aSILVA, C. G. N. da
245 $aDesigning and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant.$h[electronic resource]
260 $c2023
520 $aEndophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8 T ), Gluconacetobacter diazotrophicus (Pal5 T ), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specific primers for the real-time PCR (qPCR) assay. Primer specificity was confirmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the five target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specified in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplified target DNA fragments from each of the bacterial species, enabling their differentiation at the species level. The total bacterial population present in the sugarcane quantified using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes influence the endophytic population and the bacterial number decreases with plant age
653 $aDiazotrophic bacteria
653 $aDPGPB quantification
653 $aPrimers
653 $aReal Time PCR
700 1 $aMONTEIRO, E. de C.
700 1 $aDINIZ, P. P.
700 1 $aTERRA, L. A.
700 1 $aSCHWAB, S.
700 1 $aREIS, V. M.
700 1 $aARAUJO, J. L. S. de
700 1 $aURQUIAGA, S.
773 $tBrazilian Journal of Microbiology,, Published: 16 October 2023
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca |
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| Registros recuperados : 12 | |
| 5. |  | BRAGA, J. G. B.; NOVAIS, C. B. de; DINIZ, P. P.; ARAGÃO, O. O. da S.; SAGGIN JUNIOR, O. J.; JESUS, E. da C. Association of mycoheterotrophic Gentianaceae with specific Glomus lineages. Mycorrhiza, v. 33, n. 4, p. 249 - 256, July 2023.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 6. | | AGUIAR, P. L.; DINIZ, P. H. S.; COSTA, L. R.; RATTES, R. A.; LOPES, A. T.; LEITAO, R. C.; BARCELLOS, W. M. Performance evaluation of biogas fueled generator set. Journal of the Brazilian Society of Mechanical Sciences and Engineering, v. 43, n. 9, art. no. 409, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 7. | | SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. Designing and validation of specifc primers for the quantitative detection of bacteria in sugarcane inoculant. Brazilian Journal of Microbiology, v. 54, p. 2627–2640, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 8. | | SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant. Brazilian Journal of Microbiology,, Published: 16 October 2023| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 9. | | LIMA, C. M. de; FERNANDES, D. D. S.; PEREIRA, G. E.; GOMES, A. de A.; ARAÚJO, M. C. U. de; DINIZ, P. H. G. D. Digital image-based tracing of geographic origin, winemaker, and grape type forred wine authentication. Food Chemistry, v. 312, p. 12606, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 10. | | RABELO, B. dos S.; VAN LANGEVELDE, F.; TOMLINSON, K.; DINIZ, P.; SILVA, D. A. da; BARBOSA, E. R. M.; BORGHETTI, F. Effects of native and invasive grasses on the survival and growth of tree seedlings in a neotropical savanna. Biological Invasions, v. 25, p. 2697-2711, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | RAMOS, D. R.; LIAFFA, A. B. S.; DINIZ, P.; MUNHOZ, C. B. R.; OOI, M. K. J.; BORGHETTI, F.; VALLS, J. F. M. Seed tolerance to heating is better predicted by seed dormancy than by habitat type in Neotropical savanna grasses. International Journal of Wildland Fire, v. 25, 1273-1280, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | FRADE, M. T. S.; DINIZ, P. V. N.; OLINDA, R. G.; MAIA, L. A.; GALIZA, G. J. N. de; SOUZA, A. P. de; NÓBREGA NETO, P. I. da; DANTAS, A. F. M. Pythiosis in dogs in the semiarid region of Northeast Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 5, p. 485-490, maio 2017. Título em português: Pitiose em cães na região semiárida do Nordeste, Brasil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 12 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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